Biology Biotechnology Principles and Processes Single Correct MCQ
Published on: August 20, 2026

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A
When cut by the same restriction enzyme, the resultant DMA fragments have the same kind of ______ and these can be joined together end-to-end using - sticky ends, recombinase; - sticky ends, DNA ligases; - sticky ends, DNA ligases; - sticky ends, DNA ligases;
B
Disruption of the cell membranes can be achieved by treating the bacterial cells plant or animal tissue with enzymes such as _____ (bacteria), _____ (plant cells) and _____ (fungus). - lysozyme, cellulase, chitinase; - exonuclease, cellulase, chitinase; - lysozyme, cellulase, chitinase; - lysozyme, cellulase, chitinase;
C
If any protein encoding gene is expressed in a heterologous host, it is called a _____. - recombinant protein; - recombinant protein; - native protein; - recombinant protein;
D
In gel electrophoresis, the separated DMA fragments can be seen only after staining the DNA with a compound known as ______ followed by exposure to UV radiation. – ethidium bromide – ethidium bromide - caesium chloride – ethidium Bromide

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D

Statement : When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of sticky ends, and these can be joined together end-to-end using DNA ligases.

Statement : Disruption of the cell membranes can be achieved by treating the bacterial cells, plant, or animal tissue with enzymes such as lysozyme (bacteria), cellulase (plant cells), and chitinase (fungus).

Statement : If any protein-encoding gene is expressed in a heterologous host, it is called a recombinant protein.

Statement : In gel electrophoresis, the separated DNA fragments can be seen only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation.

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